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61.
Mehedi Hassan Aishwarya Alex Namasivayam Dan DeBlasio Nazeefa Fatima Benjamin Siranosian R. Gonzalo Parra Bart Cuypers Sayane Shome Alexander Miguel Monzon Julien Fumey Farzana Rahman 《BMC bioinformatics》2018,19(12):347
This article describes the motivation, origin and evolution of the student symposia series organised by the ISCB Student Council. The meeting series started thirteen years ago in Madrid and has spread to four continents. The article concludes with the highlights of the most recent edition of annual Student Council Symposium held in conjunction with the 25th Conference on Intelligent Systems for Molecular Biology and the 16th European Conference on Computational Biology, in Prague, in July 2017. 相似文献
62.
Alien fishes are considered a major threat to aquatic biodiversity in South Africa, yet relatively little regional information on their biology and ecology is available for many of these species. Seasonal changes in the diet of the bluegill Lepomis macrochirus in Howieson’s Poort Dam, Grahamstown, were assessed during summer and winter in 2014–2015, using stomach content analysis. In winter, juvenile and adult fish diets were dominated by crustacean zooplankton and insects, respectively. In summer, juvenile fish fed on crustaceans and insects, whereas adults consumed mostly fish eggs, indicating a potential impact by these invasive fish on native fish through oophagy. 相似文献
63.
64.
Molecular phylogenetic and biogeochemical studies of sulfate-reducing bacteria in the rhizosphere of spartina alterniflora 总被引:1,自引:0,他引:1
Hines ME Evans RS Sharak Genthner BR Willis SG Friedman S Rooney-Varga JN Devereux R 《Applied and environmental microbiology》1999,65(5):2209-2216
The population composition and biogeochemistry of sulfate-reducing bacteria (SRB) in the rhizosphere of the marsh grass Spartina alterniflora was investigated over two growing seasons by molecular probing, enumerations of culturable SRB, and measurements of SO42- reduction rates and geochemical parameters. SO42- reduction was rapid in marsh sediments with rates up to 3.5 &mgr;mol ml-1 day-1. Rates increased greatly when plant growth began in April and decreased again when plants flowered in late July. Results with nucleic acid probes revealed that SRB rRNA accounted for up to 43% of the rRNA from members of the domain Bacteria in marsh sediments, with the highest percentages occurring in bacteria physically associated with root surfaces. The relative abundance (RA) of SRB rRNA in whole-sediment samples compared to that of Bacteria rRNA did not vary greatly throughout the year, despite large temporal changes in SO42- reduction activity. However, the RA of root-associated SRB did increase from <10 to >30% when plants were actively growing. rRNA from members of the family Desulfobacteriaceae comprised the majority of the SRB rRNA at 3 to 34% of Bacteria rRNA, with Desulfobulbus spp. accounting for 1 to 16%. The RA of Desulfovibrio rRNA generally comprised from <1 to 3% of the Bacteria rRNA. The highest Desulfobacteriaceae RA in whole sediments was 26% and was found in the deepest sediment samples (6 to 8 cm). Culturable SRB abundance, determined by most-probable-number analyses, was high at >10(7) ml-1. Ethanol utilizers were most abundant, followed by acetate utilizers. The high numbers of culturable SRB and the high RA of SRB rRNA compared to that of Bacteria rRNA may be due to the release of SRB substrates in plant root exudates, creating a microbial food web that circumvents fermentation. 相似文献
65.
Codon fitnesses for chloroplast genes were estimated using the relative
synonymous codon use of psbA, which has a different pattern of codon use
than other chloroplast genes and is the major translation product of the
chloroplast. These estimates were used to calculate the codon adaptation
index (CAI) of chloroplast genes from Marchantia polymorpha, Nicotiana
tabacum, and Chlamydomonas reinhardtii. The genes with the highest CAI
values in M. polymorpha correspond to those that are expressed at the
highest levels. The rate of divergence between M. polymorpha and both C.
reinhardtii and N. tabacum is inversely related to the CAI value of the M.
polymorpha gene. The data suggest that selection is acting on the
synonymous codon use of the highly expressed genes of the M. polymorpha
chloroplast genome. The data set is inconclusive about N. tabacum genes,
but, as there is a weaker correspondence between CAI value and expression
level, it suggests that selection is not operating in this lineage.
相似文献
66.
Dey G Palit S Banerjee R Maiti BR 《Journal of industrial microbiology & biotechnology》2002,28(4):193-200
A maltooligosaccharide-forming amylase that hydrolyzes starch into maltotriose and maltopentaose was found in the culture
filtrate of a strain of Bacillus circulans GRS 313 isolated from local soil. The enzyme was purified by organic solvent fractionation, Sephadex G-100 gel filtration
and CM-Sephadex column chromatography. Optimum pH and temperature of amylase were evaluated using response surface methodology
(RSM) and were found to be 48°C and 4.9, respectively. The enzyme was stable up to 60°C and its pH stability was in the range
of 5.0–8.0. The K
m and V
max of the amylase with starch were 11.66 mg/ml and 68.97 U, respectively, and the energy of activation, E
a, was 7.52 kcal/mol. Dextrin inhibited the enzyme competitively, with a K
i of 6.1 mg/ml, and glucose caused noncompetitive inhibition with a K
i of 9.5 mg/ml. The enzyme was inhibited by Hg2+, Mn2+, Fe3+ and Cu2+ and enhanced by Co2+ and Mg2+. EDTA reversed the inhibitory effect of the metals. Paper chromatographic and high-performance liquid chromatography analysis
of the products of the amylolytic reaction showed the presence of maltotriose, maltotetraose, maltopentaose, maltose and glucose
in the starch hydrolysate. Journal of Industrial Microbiology & Biotechnology (2002) 28, 193–200 DOI: 10.1038/sj/jim/7000220
Received 11 December 2000/ Accepted in revised form 22 October 2001 相似文献
67.
The recruitment of Raf-1 to membranes is mediated by direct interaction with phosphatidic acid and is independent of association with Ras 总被引:17,自引:0,他引:17
The serine/threonine kinase Raf-1 is an essential component of the MAPK cascade. Activation of Raf-1 by extracellular signals is initiated by association with intracellular membranes. Recruitment of Raf-1 to membranes has been reported to be mediated by direct association with Ras and by the phospholipase D product phosphatidic acid (PA). Here we report that insulin stimulation of HIRcB fibroblasts leads to accumulation of Ras, Raf-1, phosphorylated MEK, phosphorylated MAPK, and PA on endosomal membranes. Mutations that disrupt Raf-PA interactions prevented recruitment of Raf-1 to membranes, whereas disruption of Ras-Raf interactions did not affect agonist-dependent translocation. Expression of a dominant-negative Ras mutant did not prevent insulin-dependent Raf-1 translocation, but inhibited phosphorylation of MAPK. Finally, the PA-binding region of Raf-1 was sufficient to target green fluorescent protein to membranes, and its overexpression blocked recruitment of Raf-1 to membranes and disrupted insulin-dependent MAPK phosphorylation. These results indicate that agonist-dependent Raf-1 translocation is primarily mediated by a direct interaction with PA and is independent of association with Ras. 相似文献
68.
69.
Summary The paper deals with the isolation of a new predacious fungusDactylaria dasguptaii
Shome &Shome from Indian soil. The fungus has been studied both in culture and soil. The predatory, morphological and reproductive characters of the new species accompanied by latin diagnosis has been described with illustrative diagrams. 相似文献
70.
A new type of roselle (Hibiscus sabdariffa L.) differing from other types in respect of certain morphological and physiological characters is described. Characteristic glandless sepal (gs) was monofactorial recessive to the normal glanded sepal (Gs). The normal ovoid (Co) shape of capsules was incompletely dominant over the round shape (co) in the new type. No linkage was observed between gs and co. 相似文献